A guide for the laboratory use of zebrafish danio rerio monte westerfield on. Zebrafish of the tl and wik strains were maintained according to standard procedures westerfield, 1995 and by following guidelines approved by the university of california, san francisco, institutional animal care and use committee. Safety evaluation of stacked genetically modified corn event mon89034. Dcc regulates asymmetric outgrowth of forebrain neurons in zebrafish. Both zebrafish sm22 genes are more closely related to each other when compared in a phylogram with human sm22. Pdf breeding of zebrafish in the laboratory environment. We will contact you to arrange shipments when normal operations are resumed.
This book is now in its fifth edition westerfield, 2007. However, i think my middle schoolers might enjoy this book so i. They are commonly found in slow moving water such as streams, canals, ditches, ponds, as well as rice fields and. For example, zebrafish larvae are too slow to efficiently capture artemia nauplii during the first few days of exogenous feeding and are much more adept at feeding on slower moving paramecium westerfield, 2007a, westerfield, 2007a or rotifers during that period best et al. In the last 30 years, the zebrafish has become a widely used model organism for research on vertebrate development and disease. Obesity is a major cause of type 2 diabetes mellitus t2dm in mammals. Feel free to add new protocols to the appropriate section or add comments to any existing protocol. A guide for the laboratory use of zebrafish danio rerio 4th ed ed. A paperback version of the fifth edition, called the zebrafish book. The zebrafish book a guide for the laboratory use of zebrafish danio brachydanio rerio by monte westerfield, institute of neuroscience, university of oregon older literature referred to zebrafish as brachydanio rerio. Studies of reward behaviour, learning and memory, aggression, anxiety and sleep strongly suggest that conserved regulatory processes underlie behaviour in zebrafish and mammals. Breeding of zebrafish in the laboratory environment for research development. Zebrafish is a cute book with fun illustrations and a great message. Pdf effects of stocking density on growth of zebrafish.
The need to develop standardized diets to support zebrafish danio rerio research is supported by the knowledge that specific dietary ingredients, nutrients, or antinutritional factors in diets have been shown to affect development and growth of adult d. Development of a novel zebrafish model for type 2 diabetes. Since that time, there have been additional books detailing zebrafish husbandry methods for research laboratories harper and lawrence, 2011, nussleinvolhard and dahm, 2002. A guide for the laboratory use of zebrafish danio rerio plastic comb january 1, 2007 by monte westerfield author. Pdf on apr 1, 2007, neil vargesson and others published zebrafish in manual of animal technology find, read and cite. Recent research has demonstrated the suitability of adult zebrafish to model some aspects of complex behaviour. A guide for the laboratory use of zebrafish danio rerio, 5th edition. Pdf zebrafish in manual of animal technology researchgate. A guide for the laboratory use of zebrafish danio rerio, published in 2007, may be ordered from the zebrafish international resource center, zirc. Monte westerfield is the author of zebrafish book 4. Through a powerful combination of genetics and experimental embryology, significant inroads have been made into the regulation of embryonic axis formation, organogenesis, and the development of neural networks.
Everyday low prices and free delivery on eligible orders. Zebrafish, danio rerio as a replacement alternative model useful. A highly accessible classroom aquaculture rig zebrafish. Zebrafish fabp4 and its 5upstream sequence were identified from a danio rerio dna sequence assembly deposited in genbank accession number cr759777 by the wellcome trust sanger institute. We have adapted existing protocols to optimize immunocytochemistry in zebrafish, and have tested many antibodies developed against mouse, chick, and frog muscle antigens for their crossreactivity in zebrafish. Protocols are organized into sections corresponding to the chapters of the zebrafish book, 5th edition 4th edition online. Due to the high level of orthology between the zebrafish and human genome and the many advantages related to laboratory manipulation, zebrafish have become an important research model to understand human diseaserelated genes lieschke and currie 2007. Use of zebrafish in research and importance of health. This material is from the 4th edition of the zebrafish book. In this study, there were seven dietary treatments consisting of five commercially available diets and two. All orders will be processed in the order received. For all experiments, zebrafish larvae were maintained in embryo medium consisting of 0.
A guide for the laboratory use of zebrafish danio rerio rep spiral by westerfield, monte isbn. Zebrafish, danio rerio, is a tropical fish belonging to the order cypriniformes family cyprinidae genus daniozebrafish belong to the same family as carp and minnow and are indigenous to areas within the himalayan region, including pakistan, myanmar, nepal, and india 1,2. The zebrafish danio rerio, hamilton, 1822 is a small fresh water fish s pecies broadly distributed in india, bangladesh and nepal eng eszer et a l. Highresolution in situ hybridization to wholemount. Wholebody cortisol response of zebrafish to acute net. Due to the covid19 pandemic, resource distribution has been suspended and services may be delayed. Use in studying cellular mechanisms for a spectrum of clinical disease entities chihsin hsu, zhihong wen, chanshing lin and chiranjib chakraborty. This is where zebrafish researchers can share experimental protocols and tips with the rest of the research community. A guide for the laboratory use of zebrafish danio rerio, 5th ed. A guide for the laboratory use of zebrafish danio rerio, eugene, university of oregon press. A guide for the laboratory use of zebrafish danio rerio, published in 2007, may be ordered from the zebrafish international. Dmso effects larval zebrafish danio rerio behavior, with additive. A guide for the laboratory use of zebrafish brachydanio rerio monte westerfield. Distributed by the zebrafish international resource center 2007.
A variation between the cdna sequence and the genomic dna sequence within exon 2 is highlighted in bold, with the variation indicated above. A guide for the laboratory use of zebrafish danio rerio unknown binding january 1, 2000 by monte westerfield author see all formats and editions hide other formats and editions. A guide for the laboratory use of zebrafish as want to read. The zebrafish has developed into a popular model for the study of myogenesis, and immunocytochemical techniques have been critical. Cole nj, hollway ge, berger j, currie pd 2007 the zebrafish candyfloss mutant implicates extracellular matrix adhesion failure in laminin alpha2deficient congenital muscular dystrophy. Zfin is a curated resource for zebrafish biology comprised of the following primary data types. The isolation and molecular analysis of zebrafish behavioural mutants is now starting.
A guide for the laboratory use of zebrafish danio rerio 9789994860579 by westerfield, monte and a great selection of similar new, used and collectible books available now at great prices. Immunocytochemistry to study myogenesis in zebrafish. Enter a book quantity zebrafish international resource center. Zebrafish, danio rerio, are frequently handled during husbandry and experimental procedures in the laboratory, yet little is known about the physiological responses to such stressors. However the execution felt off for me the book was a little all over the place and i found it difficult to track the dialogue and action of the story. The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. Monte westerfield author of essential zebrafish methods. An alignment of zebrafish sm22a and sm22b genes shows a moderate level of conservation. Hardcopies of the 4th edition of the zebrafish book can be obtained for a nominal fee from the zebrafish international resource center, 5274 university of oregon, eugene, or 97403 usa. The journal will serve as a forum for papers discussing research on comparative genomics and evolution, the genetic analysis of embryogenesis and disease and the.
Phosphorylation of the zebrafish m6ab at serine 263 contributes to filopodium formation in pc12 cells and neurite outgrowth in zebrafish embryos. The laboratory zebrafish crc press book like other books in the laboratory animal pocket reference series, this guide covers all aspects pertaining to the use of these organisms including their basic biology, humane care and management, husbandry, life support systems, regulatory compliance, technical procedures, veterinary care, and wate. We have previously established a zebrafish model of dietinduced obesity dio zebrafish by overfeeding artemia. Spatiotemporal expression of smooth muscle markers in. The fabp4 gene of zebrafish danio rerio febs press. Jingxia gao, changwen zhang, bin yang, liu sun, cuizhen zhang, monte westerfield, gang peng. Adult zebrafish as a model organism for behavioural. Solvents are frequently used during zebrafish toxicity testing but their effects. A guide for the laboratory use of zebrafish danio rerio. In the whitesnake mutant, a brain phenotype is visible by 28 hr after fertilization, when it becomes apparent that the midbrain and hindbrain are abnormally shaped. We measured the wholebody cortisol levels of adult zebrafish subjected to net stress and air exposure at intervals over a 24 h period. A guide for the laboratory use of zebrafish danio brachydanio rerio. Zebrafish danio rerio and its embryo has become a popular replacement.